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Diagnostic Microsatellite Markers for the Detection of Stem Rust Resistance Gene Sr36 in Diverse Genetic Backgrounds of Wheat

^a Dep. of Agronomy and Plant Genetics, 411 Borlaug Hall, Univ. of Minnesota, St. Paul, MN 55108
^b USDA-ARS, Cereal Disease Lab., 1551 Lindig Ave., Univ. of Minnesota, St. Paul, MN 55108

View larger version (89K): [in this window] [in a new window]   Figure 1. Gel electrophoresis showing segregation pattern of the four SSR markers, (A) Xgwm429, (B) Xgwm319, (C) Xwmc477, and (D) Xstm773-2, in a subset of the F2 progenies from a cross between near-isogenic lines (Sr36/9*LMPG and LMPG); P1, resistant parent; P2, susceptible parent; R, resistant F2; S, susceptible F2; H, heterozygous F2 progenies. The arrow points indicate the size of the band associated with Sr36.

View larger version (17K): [in this window] [in a new window]   Figure 2. Partial genetic linkage maps of chromosome 2BS depicting the location of Sr36 with linked codominant simple sequence repeat loci in the LMPG x Sr36/9*LMPG population and the CS x W2691Sr36-1 population. The linkage maps were constructed using map distances (cM) from Kosambi.