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Published online 1 January 2007
Published in Crop Sci 47:S-3-S-13 (2007)
© 2007 Crop Science Society of America
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Physical Mapping of Wheat and Rye Expressed Sequence Tag–Simple Sequence Repeats on Wheat Chromosomes

Amita Mohan, Aakash Goyal, Ravinder Singh, Harindra S. Balyan and Pushpendra K. Gupta*

Molecular Biology Lab., Dep. of Genetics and Plant Breeding, Ch. Charan Singh Univ., Meerut- 250 004 (U.P.), India


Figure 1
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  		Figure 1. PCR profiles showing strategy followed for deletion bin mapping: (a) Physical mapping of wEST–SSR PK123 (TC67549) on centromeric bin of 6DL (notice amplification in all the deletion stocks), Lanes M 100-bp ladder, 1 CS, 2 N6DT6A, 3 N6DT6B, 4 Dt6DS, 5 Dt6DL, 6 6DL-6, 7 6DL-1, 8 6DL-12, 9 6DL-11, 10 6DL-10. (b) Physical mapping of rEST–SSR SCM141 (BE495388) on interstitial bin defined by deletion breakpoints 5AL-10 and 5AL-17 (notice amplification in only some deletion stocks); Lanes M 100-bp ladder, 1 CS, 2 N5AT5B, 3 N5AT5D, 4 Dt5AL, 5 5AS-1, 6 5AS-3, 7 5AS-7, 8 5AS-10, 9 5AL-12, 10 5AL-10, 11 5AL-17, 12 5AL-23. (c) Physical mapping of wEST–SSR PK9 (BG274944) in distal bin of 2DS (notice absence of amplification in all the deletion stocks); Lanes M 100-bp ladder, 1 CS, 2 N2DT2A, 3 N2DT2B, 4 Dt2DS, 5 Dt2DL, 6 2DS-1, 7 2DS-5.

 

Figure 2
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Figure 2
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  		Figure 2. Physical mapping of wheat and rye EST–SSRs on 21 wheat chromosomes. The accession numbers of ESTs, which correspond mapped EST–SSRs are shown on the right and the fraction lengths are given on the left. Vertical lines on the right side span the bin length. The accession numbers in blue belong to wheat EST–SSRs, and those in red belong to rye EST–SSRs. The EST–SSRs, which could not be assigned to bins and were assigned to chromosome arms only are underlined and those assigned to chromosomes (with no information about arm) are given under each individual chromosome.

 

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  		Figure 3. Rice chromosomes showing physical positions of SSR-containing ESTs, mapped through homology search.

 




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