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Evaluation of Target Preparation Methods for Single-Feature Polymorphism Detection in Large Complex Plant Genomes

^a Dep. of Plant Breeding and Genetics, Institute for Genomic Diversity, Cornell Univ., 175 Biotechnology Building, Ithaca, NY 14853
^b USDA-ARS, Cornell Univ., 741 Rhodes Hall, Ithaca, NY 14853
^c Keygene N.V., Agro Business Park 90, P.O. Box 216, 6700 AE Wageningen, The Netherlands
^d School of Forest Resources and Conservation, Univ. of Florida, Gainesville, FL 32610
^e USDA-ARS and Dep. of Plant Breeding and Genetics, Institute for Genomic Diversity, Cornell Univ., 159 Biotechnology Building, Ithaca, NY 14853
^f contributed equally to this work

View larger version (15K): [in this window] [in a new window]   Figure 1. Frequency distribution of perfect match (PM) and mismatch (MM) probe pair signal intensity ratios. Probe pair signal intensity ratios are shown according to log2PM range for amplified fragment length polymorphism (AFLP), mRNA, Cot filtration (CF), and methylation filtration (MF).