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Published online 7 November 2007
Published in Crop Sci 47:2445-2452 (2007)
© 2007 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Effect of Temperature on Microsomal Omega-3 Linoleate Desaturase Gene Expression and Linolenic Acid Content in Developing Soybean Seeds

Grace E. Byfielda and Robert G. Upchurchb,*

a Microbiology Dep., North Carolina State Univ., Raleigh, NC 27695
b USDA-ARS, Soybean and Nitrogen Fixation Unit and Plant Pathology Dep., North Carolina State Univ., Raleigh, NC 27695


Figure 1
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  		Figure 1. Effect of temperature (day/night) on mean oleic, linoleic, and linolenic acid content (mg g–1 oil) of developing soybean seeds of Dare, N99-3170, and N01-3544 at 35 d after flowering (DAF). Values represent mean ± SE (p < 0.05).

 

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  		Figure 2. Selected gas chromatography traces showing the effect of temperature (day/night [D/N]) on seed fatty acid composition at 35 d after flowering: left to right, cool (D/N = 22/18°C); normal (D/N = 26/22°C); warm (D/N = 30/26°C). (A, B, C) Soybean cultivar Dare; (D, E, F) soybean variety N99-3170; (G, H, I) soybean variety N01-3544.

 

Figure 3
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  		Figure 3. Effect of temperature (day/night) on the mean expression of the GmFAD3 genes in developing soybean seeds of varieties Dare, N99-3170, and N01-3544 at 35 d after flowering. Values are normalized to actin and represent mean ± SE (p < 0.05).

 




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