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Molecular Mapping of the Russian Wheat Aphid Resistance Gene Dn2414 in Wheat

^a Lapitan, Soil and Crop Sciences Dep., Colorado State Univ., Fort Collins, CO 80523-1170
^b current address: Wuhan Botanical Garden/Institute, The Chinese Academy of Sciences, Wuhan, Hubei 430074, China
^c Dep. Bioagricultural Sciences and Pest Mgmt., Colorado State Univ., Fort Collins, CO 80523-1177

View larger version (84K): [in this window] [in a new window]   Figure 1. Amplification profiles of polymerase chain reaction (PCR)-based markers in the Dn2414 mapping population. (a) Bulked segregant analyses of four markers, Xgwm11, Xgwm18, Xrems1303, and Xiag95. Lanes 1 to 4 represent 2414-11, ‘Yuma’, resistant bulk, and susceptible bulk, respectively. Panels (b), (c), and (d) show the PCR patterns of Xgwm18, Xrems1303, and Xiag95, respectively. Lanes P1 and P2 represent 2414-11 and Yuma, respectively. The black or white arrows indicate the diagnostic band of specific markers. Lanes 1 to 23 represent 23 F2 individual plants. The genotypes of RWA resistance gene Dn2414 are shown for specific lanes/individuals: RR = homozygous resistant, Rr = heterozygous resistant, rr = homozygous susceptible.

View larger version (11K): [in this window] [in a new window]   Figure 2. High-density polymerase chain reaction (PCR)-based genetic map of rye chromosome 1R short arm (1RS).1BL (long arm of wheat chromosome 1B) chromosome carrying Russian wheat aphid (RWA) resistance gene Dn2414. The interval length (cM) is indicated on the left side, and marker order and names are shown on the right side of the genetic map.

View larger version (85K): [in this window] [in a new window]   Figure 3. Multiplex polymerase chain reaction (PCR) amplification patterns of Xrems1303 + Xgwm18 in 46 wheat cultivars, accessions, and breeding lines. The black or white arrows indicate the diagnostic band of specific markers. The lane codes are corresponding to that of cultivars or lines shown in Table 4. Resistant genotypes are shown based on the infestation results of biotype 2 Russian wheat aphid (RWA): RR = homozygous resistant, Rr = heterozygous resistant, rr = homozygous susceptible. The size difference among Xgwm18 bands reflects polymorphism among the wheat cultivars or recurrent parents of backcross-derived breeding lines.