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Published online 1 September 2007
Published in Crop Sci 47:2067-2073 (2007)
© 2007 Crop Science Society of America
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Correlation between Heat Stability of Thylakoid Membranes and Loss of Chlorophyll in Winter Wheat under Heat Stress

Zoran Ristica,*, Urska Bukovnikb and P.V. Vara Prasadb

a USDA-ARS, Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Manhattan, KS 66506
b Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506


Figure 1
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Figure 1. (A) The ratio of constant fluorescence and the peak of variable fluorescence (O/P) and (B) chlorophyll content in flag leaves from 12 cultivars of winter wheat under heat stress conditions. Chlorophyll a fluorescence and chlorophyll contents were measured on the same flag leaves after 0, 8, 10, 12, 14, and 16 d of exposure to heat stress. Increases in O/P indicate damage to thylakoid membranes, the greater the damage the lower the tolerance to heat stress (Ristic and Cass, 1993). Plotted data are from Experiment 1. Bars indicate ±standard errors; n = 5. Similar results were observed in a duplicate experiment.

 

Figure 2
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Figure 2. Correlation between the ratio of constant fluorescence and the peak of variable fluorescence (O/P) and chlorophyll content expressed as percentage of control (plants not exposed to heat) in the flag leaf from 12 cultivars of winter wheat. Data from five replicate plants of each cultivar were averaged and used for correlation analysis. Plotted data are from Experiment 1; n = 12. Similar results were obtained in a duplicate experiment. HS, heat stress.

 

Figure 3
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Figure 3. Correlation between the ratio of constant fluorescence and the peak of variable fluorescence (O/P) and chlorophyll content in the flag leaves of 12 cultivars of winter wheat. Data represent an average of two experiments in which in each experiment data from five replicate plants of each cultivar were averaged. (A) The chlorophyll content in heat-stressed plants, measured on Days 8, 10, 12, 14, and 16 of heat treatment, was expressed as percentage of that in control (plants not exposed to heat stress). (B) The chlorophyll content in heat-stressed plants, measured on Days 8, 10, 12, 14, and 16 of heat treatment, was expressed as percentage of chlorophyll content measured in the same plants at the beginning of heat stress (Day 0 of heat stress). Data for all days of heat stress treatment when fluorescence and chlorophyll content were measured are plotted on the same graph (n = 60).

 





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