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Marker-Assisted Selection of Columbia Root-Knot Nematode Resistance Introgressed from Solanum bulbocastanum

^a IAREC, Washington State Univ., 24106 N. Bunn Rd., Prosser, WA 99350
^b USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350
^c USDA-ARS and Univ. of California-Berkeley, Plant Gene Expression Center, 800 Buchanan St., Albany, CA 94710

View larger version (10K): [in this window] [in a new window]   Figure 1. Scheme of introgression of Meloidogyne chitwoodi resistance into a tetroploid gene pool. SB22 = resistant; CBP233 = F1 (through protoplast fusion with R4), A84118–3, tetraploid breeding lines.

View larger version (10K): [in this window] [in a new window]   Figure 2. Potato Chromosome 11 of the parent SB22 and the markers tightly linked to the Columbia root-knot nematode resistance gene RMc1(blb) on the distal end of the upper arm of potato Chromosome 11. Distance is measured in centimorgans.

View larger version (88K): [in this window] [in a new window]   Figure 3. Segregation of sequence tagged site (STS) markers (a) 39E18 and (b) 193I9 in BC5 breeding lines derived from the cross between a backcross resistant parent and susceptible cultivated potato. Individuals with RMc1(blb) nematode resistance locus contain a band of 400 base pairs (bp) in 39E18 and 300 bp in 193I9. R = resistant, S = susceptible.