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Microsatellite Markers Linked to Stem Rust Resistance Allele Sr9a in Wheat

^a Dep. of Agronomy and Plant Genetics, 411 Borlaug Hall, Univ. of Minnesota, St. Paul, MN 55108
^b USDA-ARS Cereal Disease Lab., 1551 Lindig Ave, Univ. of Minnesota, St. Paul, MN 55108

View larger version (66K): [in this window] [in a new window]   Figure 1. Polyacrylamide gel electrophoresis showing the segregation pattern of the four microsatellite markers (A) Xgwm47, (B) Xgwm120, (C) Xbarc101, and (D) Xwmc175 in a subset of the F2 progenies from a cross between Sr9a near-isogenic lines (‘Chinese Spring’ and ISr9a-Ra); M is a 10-base pair (bp) ladder, 1 is resistant parent ISr9a-Ra, 2 is susceptible parent Chinese Spring, R is resistant F2 progeny, S is susceptible F2 progeny, and H is heterozygous resistant F2 progeny. Arrowheads indicate the size of the band associated with the Sr9a gene in coupling linkage (A–C) and also in repulsive linkage (D). Recombination or crossing over between the marker allele and the resistance allele is indicated by X.

View larger version (13K): [in this window] [in a new window]   Figure 2. A genetic linkage map of the Sr9a locus and the linked simple sequence repeat markers on the long arm of chromosome 2BL. The linkage map was constructed using 116 F2 individuals derived from a cross between near-isogenic lines (‘Chinese Spring’ and ISr9a-Ra). Names of markers and the gene are shown on the right.