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Expansion of PCR-based Marker Resources in Oat by Surveying Genome-Derived SSR Markers from Barley and Wheat

USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1691 South 2700 West, Aberdeen, ID 83210

View larger version (52K): [in this window] [in a new window]   Figure 1. Distribution of simple sequence repeat (SSR) primers selected from barley. Distances between markers do not reflect the exact map positions. Primer pairs in bold letters produced positive polymerase chain reaction (PCR) products in oat lines.

View larger version (39K): [in this window] [in a new window]   Figure 2. Distribution of simple sequence repeat (SSR) primers selected from wheat. Distances between markers do not reflect the exact map positions. Primer pairs in bold letters produced positive polymerase chain reaction (PCR) products in oat lines. Numbers at the top represent the specific chromosomes. A, B, and D after each number designate the different subgenomes of the chromosomes. Marker Barc59 is indicated on both chromosomes 5B and 2D.

View larger version (100K): [in this window] [in a new window]   Figure 3. Example of marker production profiles of four primer pairs in oat. The primer pairs were from barley; polymerase chain reaction (PCR) products were separated on a 6% polyacrylamide gel. The first lane is a 100-bp ladder. T, TAM-O-301; O1, Ogle1040; K, Kanota156; O2, Ogle157.