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Published online 1 September 2007
Published in Crop Sci 47:1878-1886 (2007)
© 2007 Crop Science Society of America
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Optimization of the Marker-Based Procedures for Pyramiding Genes from Multiple Donor Lines: II. Strategies for Selecting the Objective Homozygous Plant

T. Ishiia and K. Yonezawab,*

a Marker-Assisted Rice Breeding Research Team, National Institute of Crop Science, Tsukuba 305-8518, Japan
b Dep. of Biotechnology, Kyoto Sangyo Univ., Kyoto 603-8555, Japan


Figure 1
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Figure 1. Diagrammatical definition of the selection strategies to be compared. I0 represents an initial heterozygous plant (root genotype) that has been produced via a schedule of crossing between multiple donor lines (Ishii and Yonezawa, 2007). For simplicity, generations in all strategies were designated by the same symbol Gi (i = 1,..., T). Selection procedures with codominant and dominant markers were written in roman and italic letters, respectively. No difference exists between codominance and dominance in strategy recurrent haplo-diploidization and crossing (RHC). Marker genotyping may be performed for haploids, only ones with a desirable genotype being haplo-diploidized. Symbols are defined as follows: Ni = a maximum permissible number of plants genotyped in generation Gi, IG = a plant with objective homozygous marker genotype, PG = a plant with a promising genotype that has a potential to leave IG in its progeny, CGP = a pair of plants with the best complementary genotypes to leave IG, PP = a plant with a promising marker phenotype (defined when the markers are dominant), and CPP = a pair of plants with complementary marker phenotypes.

 





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