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Published online 1 September 2007
Published in Crop Sci 47:1813-1822 (2007)
© 2007 Crop Science Society of America
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Quantitative Trait Loci Identified for Resistance to Stagonospora Glume Blotch in Wheat in the USA and Australia

J. Uphausa, E. Walkerb, M. Shankarb, H. Golzarb, R. Loughmanb, M. Franckib and H. Ohma,*

a Dep. of Agronomy, Lilly Hall, Purdue Univ., 915 W. State St., West Lafayette, IN 47907
b Dep. of Agriculture and Food Western Australia, Locked Bag 4, Bentley Delivery Centre, WA, Australia. M. Francki also Value Added Wheat Cooperative Research Centre, North Ryde, NSW, 2113 Australia. Contribution from Purdue Univ. Agricultural Research Programs as Journal Article no. 2005-17714


Figure 1
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Figure 1. Frequency distributions of stagonospora nodorum blotch (SNB) glume disease severity in a wheat (Triticum aestivum L.) recombinant-inbred population derived from the cross of the Purdue University breeding lines P91193D1 and P92201D5 evaluated at (A) southern Indiana, (B) Lafayette, IN, and (C) South Perth, Australia, and in greenhouse experiments in (D) 2003 and (E) 2004.

 

Figure 2
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Figure 2. Composite interval analysis of chromosome 2D for the quantitative trait loci associated with stagonospora nodorum blotch (SNB) glume resistance in a wheat (Triticum aestivum L.) recombinant-inbred population derived from the cross of the Purdue University breeding lines P91193D1 and P92201D5 evaluated at (A) southern Indiana, (B) Lafayette, IN, and (C) South Perth, Australia (solid line represents SNB and dashed line represents plant height), and in greenhouse experiments in (D) 2003 and (E) 2004. The significant logarithm of odds (LOD) thresholds above each graph are indicated by * and **, signifying {alpha} = 0.05 and {alpha} = 0.01, respectively. The two linkage groups were evaluated with composite interval mapping as unlinked groups but are presented in the figures as the same linkage group. Genetic distances are given in centimorgans (cM) and markers with significant segregation distortion are indicated by * (0.05 ≥ P > 0.01) and ** (P ≤ 0.01).

 

Figure 3
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Figure 3. Polymerase chain reaction amplifications of (A) Xgwm526 from DNA of wheat (Triticum aestivum L.) by nullisomic–tetrasomic analysis and separation of products on a 12% polyacrylamide gel electrophoresis (chromosomal assignment of marker loci are shown to the left of the figure); and of (B) Xgwm526 and (C) Xcfd50 from DNA from selected wheat lines resistant (R), moderately resistant (MR), and susceptible (S) to stagonospora nodorum glume blotch (the marker loci designations are shown to the right of the figures).

 





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