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A Modified Colorimetric Method for Phytic Acid Analysis in Soybean

^a Dep. of Crop and Soil Environmental Sciences, Virginia Tech., Blacksburg, VA, 24061
^b Dep. of Plant Pathology, Physiology and Weed Science, Virginia Tech., Blacksburg, VA, 24061
^c USDA-ARS and Crop Science Dep., North Carolina State Univ., Raleigh, NC 27695

View larger version (29K): [in this window] [in a new window]   Figure 1. Correlation between seed phytic acid P content of 42 soybean genotypes determined by the modified colorimetric method and by the other assay methods: high-performance liquid chromatography (HPLC), anion exchange column (AEC), and nuclear magnetic resonance (NMR). The solid line (y = x) represents a 1:1 relationship between colorimetric phytic acid P and phytic acid P determined by other methods.

View larger version (25K): [in this window] [in a new window]   Figure 2. Mean and range of seed phytic acid P content determined by the modified colorimetric, high-performance liquid chromatography (HPLC), anion exchange column (AEC), and 31P nuclear magnetic resonance (NMR) methods for 42 soybean genotypes. NMR-1 = phytic acid P (IP6) content determined by the 31P NMR method; NMR-2 = phytic acid P (IP6) and other inositol phosphates (IP1, IP2, IP3, IP4, and IP5) determined by the 31P NMR method.

View larger version (28K): [in this window] [in a new window]   Figure 3. Scatterplot of seed inorganic P (Pi) content of the 42 soybean genotypes measured by the anion exchange column (AEC) method corresponding to their phytic acid P content as measured by different methods, colorimetric, high-performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR).

View larger version (24K): [in this window] [in a new window]   Figure 4. Ranking of phytic acid P content among 11 soybean genotypes with the modified colorimetric, high-performance liquid chromatography (HPLC), anion exchange column (AEC), and 31P nuclear magnetic resonance (NMR) methods.

View larger version (19K): [in this window] [in a new window]   Figure 5. Linear regression relationship between anion exchange column (AEC)–inorganic P and phytic acid P associated with each analytical method. The arrows represent a difference of 1 mg g–1 phytic acid P between the indicated assay methods.