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Single Nucleotide Polymorphism Detection of the Rcs3 Gene for Resistance to Frogeye Leaf Spot in Soybean

^a The Univ. of Georgia Center for Applied Genetic Technologies, Athens, GA 30602
^b Dep. of Plant Pathology, Univ. of Georgia–Griffin Campus, Griffin, GA 30223; M. Missaoui, current address: Monsanto Co., 1551 Hwy. 210, Huxley, IA50124

View larger version (22K): [in this window] [in a new window]   Figure 1. Integrated single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) map of the segment of linkage group (LG-J) containing the Rcs3 gene conferring resistance to frogeye leaf spot in soybean. The map on the left represents the USDA consensus map (http://soybase.org/; verified 24 Apr. 2007).

View larger version (9K): [in this window] [in a new window]   Figure 2. Results of direct hybridization assay for the F2 population of Davis x Blackhawk. Genotypes are represented by homozygous for the Davis allele (), heterozygous (), and homozygous for the Blackhawk allele () determined by the single base extension (SBE) assay. Numbers on the x- and y-axis represent mean fluorescent intensity (MFI).