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Breeding System of the Aerial Flowers in an Amphicarpic Clover Species: Trifolium polymorphum

^a Forage Legume Dep., National Institute of Agricultural Research, INIA Tacuarembó, Ruta 5 Km 386, Tacuarembó, Uruguay
^b Cooperative Research Centre for Plant-Based Management of Dryland Salinity, Univ. of Western Australia, University Field Station, 1 Underwood Ave., Shenton Park, WA 6009, Australia
^c School of Plant Biology, Faculty of Natural and Agricultural Sciences, Univ. of Western Australia, 35 Stirling Hwy., Crawley, WA 6009, Australia
^d Biotechnology Unit, National Institute of Agricultural Research, INIA Las Brujas, Ruta 48 Km 10, Canelones, Uruguay
^e Dep. of Agronomy, Univ. of Florida, Gainesville, FL 32611-0500

View larger version (18K): [in this window] [in a new window]   Figure 1. Qualitative yield score (1–9) of 640 T. polymorphum plants measured on 14 Dec. 1998 and 25 Jan. 1999 at INIA Tacuarembó.

View larger version (80K): [in this window] [in a new window]   Figure 2. Microsatellite marker TRSSRA01H11 amplification on T. polymorphum genotypes. Three plants belonging to nine patches. Molecular ladder on the right.

View larger version (137K): [in this window] [in a new window]   Figure 3. (A) Microsatellite marker TRSSRA01H11 amplification on 40 plants of T. polymorphum corresponding to patches J9 and J10 (1–20 = 20 plants from J9; 21–40 = 20 plants from J10; 41 = DNA ladder, 100 bp [Fermentas, Hanover, MD]). (B) Microsatellite marker TRSSRA01H11 amplification on 16 plants of T. polymorphum corresponding to patch J6 (molecular weight marker on the right, DNA ladder, 100 bp [Fermentas]).

View larger version (54K): [in this window] [in a new window]   Figure 4. Microsatellite marker TRSSRA01H11 amplification on 22 progenies of patch J10 that had leaf marks. Molecular ladder on the left (DNA ladder, 100 bp [Fermentas, Hanover, MD]).