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Published online 30 July 2007
Published in Crop Sci 47:1375-1383 (2007)
© 2007 Crop Science Society of America
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Genetic Diversity among Sorghum Races and Working Groups Based on AFLPs and SSRs

Ramasamy Perumala,*, Renganayaki Krishnaramanujamb, Monica A. Menzb, Seriba Katiléa, Jeff Dahlbergc, Clint W. Magilla and William L. Rooneyb

a Dep. of Plant Pathology and Microbiology, Texas A&M Univ., College Station, TX 77843-2132
b Dep. of Soil and Crop Science, Texas A&M Univ., College Station, TX 77843-2474
c National Sorghum Producers, 4201 N. Interstate 27, Lubbock, TX 79403


Figure 1
Figure 1
Figure 1
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  		Figure 1a, b, and c. AFLP amplification products from 46 Sorghum bicolor isolates using EcoRI-TGA/MseI-CAA (Fig. 1a) PstI-GAT/MseI-CTA (Fig. 1b) and SSR–Xgap 236 (Fig. 1c) primers as detected using a LI-COR system. (Lanes 1 to 46 represent Sorghum bicolor lines IS3620C, BTx623, SC195, SC614, SC1103, SC214, SC224, SC950, SC15, SC315, SC319, SC635, SC641, SC647, SC663, SC671, SC322, SC258, SC66, SC115, SC237, SC52, SC93, SC392, SC407, SC749, SC411, SC418, SC228, SC171, SC425, SC233, SC193, SC465, SC910, SC166, SC131, SC199, SC209, SC303, SC06, SC1156, SC941, RTx 436, RTx 430, and BTx 378). Ladder sizes are given in base pairs (bp).

 

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  		Figure 2. Dendrogram of 46 Sorghum bicolor lines using AFLP and SSR markers as per unweighted pair group method with arithmetic average clustering. Numbers shown at different nodes represent percentage confidence limits obtained in the bootstrap analysis and are shown for clusters with > 60%. The scale shown below is the measure of genetic similarity coefficients calculated according to Jaccard (1908).

 

Figure 3
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  		Figure 3. Two-dimensional picture of principal coordinate analysis estimated using AFLP and SSRs genetic similarity matrix of 46 Sorghum bicolor lines.

 




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