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Identification of Molecular Markers Associated with Root-Knot Nematode Resistance in Upland Cotton

^a Dep. of Plant and Environ. Sci., Box 30003, New Mexico State Univ., Las Cruces, NM 88003
^b USDA-ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124
^c Cotton Incorporated, 6399 Weston Pkwy., Cary, NC 27513
^d Dep. of Nematology, Univ. of California, Riverside, CA 92521-0415

View larger version (37K): [in this window] [in a new window]   Figure 1. Generation of a DNA marker associated with cotton resistance to root-knot nematode. DNA samples prepared from three pairs of cotton NIL were subjected to RAPD analysis using the decamer primers UBC693 (A) and UBC781 (B). Products in (A) were separated on a 1.2% (w/v) agarose gel, stained with EtBr and products in (B) were separated on a 5% polyacrylamide gel and visualized by silver staining. The approximately 420-bp band in (A) and 1000-bp band in (B) are present only in the lanes representing the RKN resistant lines and indicated by the arrows.

View larger version (31K): [in this window] [in a new window]   Figure 2. STS primers designed from the polymorphic 1000-bp amplification product generated by the RAPD primer UBC781 amplified a 964-bp UBC781 STS marker. The UBC781-STS marker is absent in all susceptible lines and present in some of the resistant lines.

View larger version (64K): [in this window] [in a new window]   Figure 3. Gel image of amplification products representing the markers UBC781-STS (A), UBC693 (B), and UCR-STS (C) in part of an F2 (ST 474 x Auburn 634) segregating population. The bands were resolved on agarose gels (A and B) and a polyacrylamide gel (C).

View larger version (15K): [in this window] [in a new window]   Figure 4. Location of UBC781-STS and UBC693 markers relative to the RKN resistance gene Mi2 and resistance markers reported in other studies. The linkage map was generated from an F2 (ST 474 x Auburn 634 RNR) segregating population.