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Fertility Restoration of the Sorghum A3 Male-Sterile Cytoplasm through a Sporophytic Mechanism Derived from Sudangrass

^a USDA-ARS, Chemistry Research Unit, CMAVE, c/o Dep. of Plant Pathology, 1453 Fifield Hall, Univ. of Florida, Gainesville, FL 32611
^b USDA-ARS, Grain, Forage, and Bioenergy Research Unit, c/o Dep. of Agronomy, Univ. of Nebraska–Lincoln, Lincoln, NE 68583-0937
^c Horticultural Sciences Dep., Univ. of Florida, Gainesville, FL 32611

View larger version (83K): [in this window] [in a new window]   Figure 1. Transcript analyses for enhanced mitochondrial orf107 transcript processing. Total leaf RNAs were prepared, and RNA from 2.5 g tissue was blotted to membranes and probed with the orf107 clone pHC104, which spans orf107 and carries sequences of atp9 (Tang et al., 1996b). A. S05-37, #38; B. S05-38, #40; C. S05-38, #49; D. a male-fertile BC15F2 resulting from pollination of A3Tx398 with IS1112C; E. S05-36, #176; F. S05-37, #67; and G. S05-38, #48. The 1110-, 870-, and 810-nt transcripts represent whole-length orf107 transcripts, while the 380-nt transcript results from processing. The 650-nt transcript is derived from atp9.

View larger version (48K): [in this window] [in a new window]   Figure 2. Transcript analyses for action of Mmt1. Total leaf RNAs were prepared, and RNA from 2.5 g tissue was blotted to membranes and probed with the atp4 clone pHT160 (Tang et al., 1996a). A. Male-fertile BC11F3 resulting from pollination of A3Tx398 with IS1112C; B. A3Tx398; C. S05-37, #30; D. S05-38, #40; E. S05-36, #167; F. S05-37, #167; G. S05-37, #67; and H. S05-38, #48. The population of 1044-nt transcripts is partially cleaved in the presence of Mmt1, generating the 832-nt transcript.

View larger version (79K): [in this window] [in a new window]   Figure 3. Iodine staining of pollen from self-pollinated progeny of the BC1F3 S05-37. (A) Male-fertile plant, (B) male-sterile segregant. Note similarity in size of stained and unstained pollen in (A), and low frequency of collapsed unstained pollen in (A) and (B).