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Marker-Assisted Backcrossing QTL for Partial Resistance to Sclerotinia White Mold in Dry Bean

USDA-ARS, Forage and Vegetable Crop Research Unit, 24106 North Bunn Rd., Prosser, WA, 99350. This research was supported by the National ARS Sclerotinia Initiative

View larger version (79K): [in this window] [in a new window]   Figure 1. Depiction of the codominant (sequence-characterized amplified region) SCAR marker of the Phs (phaseolin seed protein) locus run on a long 2% agarose gel for 12 h at 3V cm–1. T-phaseolin (three bands: 249, 264, and 285 bp) allele in coupling phase linkage with the B7 QTL conditioning partial resistance to white mold derived from G122 is in lane 2 = G122 donor parent, and lanes 4, 5, and 7 = partially resistant BC3F4:6 lines from Population I. S-phaseolin (two bands: 249 and 270 bp) allele linked with susceptibility is in lane 3 = Winchester recurrent parent, and lanes 6, 8, and 9 = susceptible BC3F4:6 lines from Population I. Lane 1 = 100-bp ladder.