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Soluble Peroxidase Activity in Maize Endosperm Associated with Maize Weevil Resistance

^a CIMMYT, Apdo Postal 6-641, 06600 Mexico D.F
^b Dep. of Biology, Univ. of Ottawa, Ottawa, ON, Canada, K1N 6N5
^c Dep. Ciencias de la Salud, Univ. Autonoma Metropolitana, Apdo Postal 55-5350, 09340 Mexico D.F

View larger version (83K): [in this window] [in a new window]   Figure 1. Hand sections of maize grain stained for peroxidase activity. A and C: longitudinal sections of total grain incubated with guaiacol alone (controls) demonstrating no brown staining in the endosperm (Ed), embryo (Em), or pericarp (Pr). B and D: same sections as in A and C with H2O2 added, demonstrating brown staining in the aleurone layer (Al), embryo root (Er), and scutellum (Sc). E: transverse section of radicle stained with guaiacol and H2O2 demonstrating brown staining in vascular xylem (Xi), phloem (Ph), endodermis (En), and cortex (Cx).