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Development of a PCR-Based Molecular Marker to Select for Nematode Resistance in Peanut

^a Dep. of Horticulture, Univ. of Georgia Tifton Campus, Tifton, GA 31793
^b USDA ARS, P.O. Box 748, Tifton, GA 31793. Funding was provided by the Georgia Seed Development Commission and the University of Georgia Research Foundation Cultivar Development Program

View larger version (20K): [in this window] [in a new window]   Figure 1. A 5% polyacrylamide gel separation of amplicons from COAN and Georgia Green with Primers 197/909. C, COAN; G, Georgia Green; W, water negative control; C+G, mixed and denatured products from COAN and Georgia Green.

View larger version (73K): [in this window] [in a new window]   Figure 2. Screening of the segregating population for marker RKN440 (primer 197/909). No. 1 to 45 in F2:3 families are loaded on 2% agarose gel. No. 95, NemaTAM parent; no. 96, GP-NC WS14 parent; W, water negative control; R, resistant; S, susceptible.

View larger version (14K): [in this window] [in a new window]   Figure 3. F3 individuals were amplified by primer set 909/197 to confirm the scoring for the reconstructed F2 population. Sample numbers correspond to the F2 parents. Lanes within each sample number are F3 individuals. Samples are separated on 2% agarose gel. W, water negative control; R, resistant; S, susceptible.