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Reduction of a Triticum monococcum Chromosome Segment Carrying the Softness Genes Pina and Pinb Translocated to Bread Wheat

^a Dep. of Plant Sciences, Univ. of California, Davis, CA 95616-8780
^b Dep. of Agronomy, Purdue Univ., 915 W. State St., West Lafayette, IN 47907-2054
^c current address: Instituto de Recursos Biológicos, INTA Castelar, (1712) Villa Udaondo, Buenos Aires, Argentina. M. Bonafede and L. Kong contributed equally to this work

View larger version (48K): [in this window] [in a new window]   Figure 1. BLAST (NCBI, 2006) and ClustalX (Thompson et al., 1997) sequence alignment of nucleotide sequences from (A) TmPina5A (T. monococcum Pina-Am1) and TaPina5D (T. aestivum Pina-1), (B) TmPinb5A (T. monococcum Pinb-Am1) and TaPinb5D (T. aestivum Pinb-1), and (C) TmGsp5A (T. monococcum Gsp-Am1) and TaGsp5A, 5B, 5D (T. aestivum Gsp-1). Primer sequences for sequence tagged site markers Pina-Am1, Pinb-Am1, and Gsp-Am1 are in underlined bold type. Shaded letters in DNA sequences correspond to the point mutations/insertion between T. monococcum and T. aestivum. Gel lanes: 1, DNA ladder; 2, donor of soft-textured grain (CS-5Am); 3, Chinese Spring; 4–26, BCF1 segregating population; 27, DNA ladder. Arrows indicate the T. monococcum–specific bands.

View larger version (30K): [in this window] [in a new window]   Figure 2. (A) BGGP polymerase chain reaction (PCR) products showing the 12-bp-shorter product in T. aestivum relative to the T. monococcum deletion. (B) BQ168958 PCR fragment digested with AluI. (C) BF474606 PCR product digested with MseI (3% agarose gel). (D) BG606847 dominant marker.

View larger version (21K): [in this window] [in a new window]   Figure 3. (A) Genetic map for the 5A/5Am recombinant arm, involving the Ha locus. Genetic distances are in cM; (B) Physical map of the collinear region in rice. The location of each marker in the rice physical map is indicated in parentheses (Gramene, 2006).