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Estimating the Proportion of Nitrogen Remobilization and of Postsilking Nitrogen Uptake Allocated to Maize Kernels by Nitrogen-15 Labeling

^a Station de Génétique Végétale, INRA-UPS-INAPG-CNRS, Ferme du Moulon, 91190 Gif/Yvette, France
^b Unité de Nutrition Azotée des Plantes, INRA route de St Cyr, 78026 Versailles Cedex, France
^c UMR INRA-USTL Stress abiotiques et différenciation des végétaux cultivés, Estrées-Mons, 80203 Péronne, France
^d Institut de Biotechnologie des Plantes, Université de Paris-Sud, 91405 Orsay Cedex, France

View larger version (19K): [in this window] [in a new window]   Figure 1. The expected bias in the prediction of the proportion of remobilized N according to Eq. [7] in terms of the proportion of postsilking N uptake allocated to the grain and post silking 15N uptake proportion. Graphs correspond to a true remobilization proportion of 0.65 (continuous lines) and 0.69 (broken lines). For example, with a true remobilization proportion of 0.65, a proportion of postsilking N uptake allocated to the grain of 0.80 and a postsilking 15N uptake proportion of 0.20, the bias is just 0.03. With the same proportion of postsilking N uptake allocated to the grain and a postsilking 15N uptake of 0.30, the bias is between 0.04 and 0.05.

View larger version (12K): [in this window] [in a new window]   Figure 2. Relationship between RSAgrain and RSAwhole-plant according to the stage of 15N labeling (during vegetative growth, full squares, and at silking, open circles) with the pooled data from the 2003–2004 experiment (66 genotypes); b is the slope of the regression with zero intercept.

View larger version (16K): [in this window] [in a new window]   Figure 3. Expected effect of the stage of 15N distribution on the 15N uptake and residual 15N uptake after silking. With an early stage of 15N labeling (e.g., at two adult leaf stage) the residual 15N uptake after silking could be about only 5%, whereas with a later 15N labeling (e.g., at the six-leaf stage), it could be higher than 15%.