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A Comparison of Hydroponics, Soil, and Root Staining Methods for Evaluation of Aluminum Tolerance in Medicago truncatula (Barrel Medic) Germplasm

^a The Samuel Roberts Noble Foundation, Ardmore, OK 73402
^b Dep. of Statistics, Oklahoma State University, Stillwater, OK 74078-1056

View larger version (180K): [in this window] [in a new window]   Fig. 1. Seedlings of M. truncatula accessions Jemalong A17 exposed to (a) 25 µM of Al3+ and (b) 0 µM of Al3+ for 5 d in hydroponics.

View larger version (162K): [in this window] [in a new window]   Fig. 2. Seedlings of M. truncatula accessions. (A) Jemalong A17 in limed soil; (B) Jemalong A17 in unlimed soil; (C) PI 566890 in unlimed soil; and (D) PI 566890 in limed soil grown for 7 wk.

View larger version (81K): [in this window] [in a new window]   Fig. 3. Root sections of M. truncatula accessions (A) Jemalong A17 and (B) PI 566890 exposed 25 µM of Al3+ for 5 d in hydroponics and stained with lumogallion. The more stain absorbed indicates more Al present in the root. Scale bar = 200 µm.