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Published online 6 February 2007
Published in Crop Sci 47:304-310 (2007)
© 2007 Crop Science Society of America
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Molecular Analysis of Soybean Lines with Low Palmitic Acid Content in the Seed Oil

Andrea J. Cardinala,*, Joseph W. Burtonb, Ana Maria Camacho-Rogera, Ji H. Yanga, Richard F. Wilsonb and Ralph E. Deweya

a Dep. of Crop Science, North Carolina State Univ., Raleigh, NC 27695
b USDA-ARS, Plant Sci. Res., 3127 Ligon St., Raleigh, NC 27607


Figure 2
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  		Supplemental Fig. S1. Genomic region of GmFATB1a and GmFATB1b showing the locations of gene-specific primer pairs. Sequences corresponding to exons are indicated in uppercase and intron sequences are shown in lowercase. Molecular marker FATB1a-312 is defined by primers corresponding to the sequences shown in bold type and FATB1b-314 is derived using primers corresponding to sequences that are both bolded and underlined. Marker FATB1a-411 is defined by primers corresponding to sequences in italicized type, and FATB1b-417 is generating using primers corresponding to sequences that are both italicized and underlined. Position 1 of the genomic sequences depicted corresponds to position 630 with reference to the start codons of the GmFATB1a and GmFATB1b cDNAs. The partial genomic sequences have been deposited in GenBank as Accession numbers DQ861997 (GmFATB1a) and DQ861998 (GmFATB1b).

 

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  		Fig. 1. Marker analysis of soybean lines possessing novel palmitic acid phenotypes. (A) PCR amplification products using FATB1a- and FATB1b-specific primers. Lines N79-2077 and N93-2008 are homozygous for the fapnc locus. (B) Southern blot analysis of XbaI-digested genomic DNA of the genotypes shown in (A) using GmFATB1a as the hybridization probe. Palmitic acid content for each line is indicated.

 




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