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Antioxidant Responses of Creeping Bentgrass Roots to Waterlogging

Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907

View larger version (27K): [in this window] [in a new window]   Fig. 1. Activity of (A) superoxide dismutase (SOD), (B) ascorbate peroxidase (AP), (C) glutathione reductase (GR), and (D) peroxidase (POD) as affected by 21 d of different depths of waterlogging (WL) in creeping bentgrass. The CL, WL-1, and WL-15 represent well-drained control, WL at 1 cm below the soil surface, and WL at 15 cm below the soil surface, respectively. Means followed by the same letter are not significantly different at P < 0.05. Vertical bars show ± S.E. of mean.

View larger version (33K): [in this window] [in a new window]   Fig. 2. Native gel stained for the activity of superoxide dismutase (SOD) and identification of SOD isoforms in roots of Penncross and G-6 creeping bentgrass under 21 d of waterlogging treatment (WL). Lanes 1, 2, 3 represent well-drained control (CL), WL at 1 cm below the soil surface (WL-1), and WL at 15 cm below the soil surface (WL-15), respectively. Lanes 4, 5, 6 represent CL, WL-1, and WL-15, respectively. Equal amounts of protein (65 µg) were loaded to each lane. Arrows indicate the isoforms.

View larger version (85K): [in this window] [in a new window]   Fig. 3. Native gel stained for the activity of ascorbate peroxidase (AP) isoforms in roots of Penncross and G-6 creeping bentgrass under 21 d of waterlogging treatment (WL). Lanes 1, 2, 3 represent well-drained control (CL), WL at 1 cm below the soil surface (WL-1), and WL at 15 cm below the soil surface (WL-15), respectively. Lanes 4, 5, 6 represent CL, WL-1, and WL-15, respectively. Equal amounts of protein (40 µg) were loaded to each lane. Arrows indicate the isoform bands.

View larger version (93K): [in this window] [in a new window]   Fig. 4. Native gel stained for the activity of glutathione reductase (GR) isoforms in roots of Penncross and G-6 creeping bentgrass under 21 d of waterlogging treatment (WL). Lanes 1, 2, 3 represent well-drained control (CL), WL at 1 cm below the soil surface (WL-1), and WL at 15 cm below the soil surface (WL-15), respectively. Lanes 4, 5, 6 represent CL, WL-1, and WL-15, respectively. Equal amounts of protein (25 µg) were loaded to each lane. Arrows indicate the isoform bands.

View larger version (90K): [in this window] [in a new window]   Fig. 5. Native gel stained for the activity of peroxidase (POD) isoforms in roots of Penncross and G-6 creeping bentgrass under 21 d of waterlogging treatment (WL). Lanes 1, 2, 3 represent well-drained control (CL), WL at 1 cm below the soil surface (WL-1), and WL at 15 cm below the soil surface (WL-15), respectively. Lanes 4, 5, 6 represent CL, WL-1, and WL-15, respectively. Equal amounts of protein (40 µg) were loaded to each lane. Arrows indicate the isoform bands.