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Published online 22 January 2007
Published in Crop Sci 47:219-231 (2007)
© 2007 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Pollen-Based Screening of Soybean Genotypes for High Temperatures

Mohammed A. Salema, Vijaya Gopal Kakanib, Sailaja Kotia and K. Raja Reddya,*

a Dep. of Plant and Soil Sciences, 117 Dorman Hall, Box 9555, Mississippi State Univ., Mississippi State, MS 39762
b Dep. of Agronomy, Univ. of Florida, Gainesville, FL 32611


Figure 1
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Fig. 1. Typical in vitro (A) pollen germination and (B) pollen tube length responses to temperature along with the fitted quadratic and modified bilinear lines, respectively, of four soybean genotypes (DP 5110, Hutcheson, DK5366RR, and HBK 5620RR). The symbols are recorded germination percentages and pollen tube lengths after 24 h and the lines (solid, DP 5110; long dash, Hutcheson; short dash, DK 5366RR; dots, HBK 5620RR) are predicted values by the respective fitted equations.

 

Figure 2
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Fig. 2. Relationship between (A) minimum (Tmin) and optimum (Topt) and maximum (Tmax) temperatures, and (B) Topt and Tmax for pollen germination of 44 soybean genotypes.

 

Figure 3
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Fig. 3. Response of mean (A) pollen germination and (B) pollen tube length to temperature of sensitive, intermediate, and tolerant groups of soybean genotypes based on cumulative temperature response index (CTRI).

 

Figure 4
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Fig. 4. Scanning electron microscopic images of pollen grains from pollen of three soybean genotypes, sensitive (Stalwart III, A and B), intermediate (PI 471938, C and D), and tolerant (DG 5630RR, E and F) at optimal temperature of 30/22°C (A, C, and E) and supraoptimal temperature of 38/30°C (B, D, and F). The white line in each picture is 20.00 µm in length.

 

Figure 5
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Fig. 5. Scanning electron microscopic images of pollen grains surface from pollen of three soybean genotypes, sensitive- Stalwart III (A and B), intermediate- PI 471938 (C and D) and tolerant- DG 5630RR (E and F) at optimal temperature of 30/22°C (A, C and E) and supraoptimal temperature of 38/30°C (B, D and F). The white line in each picture is 2.00 µm in length.

 





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