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Validating the Fhb1 QTL for Fusarium Head Blight Resistance in Near-Isogenic Wheat Lines Developed from Breeding Populations

Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, 411 Borlaug Hall, 1991 Buford Cir., St. Paul, MN 55108. M.O. Pumphrey, current address: Dep. of Plant Pathology, Kansas State Univ., Manhattan, KS 66506

View larger version (34K): [in this window] [in a new window]   Fig. 1. Development of quantitative trait locus–near-isogenic lines (QTL-NILs) for testing QTL effects in diverse genetic backgrounds. In Step 1, heterogeneous F4 lines are identified after genotyping a bulk harvest of F3:4 plants with DNA markers flanking the QTL of interest. In Steps 2 through 5, progeny screening of heterozygous plants produces F4–derived QTL-NILs that are then increased. The + and – symbols indicate the allelic status of the markers for the QTL, with + representing the marker for the allele under selection and – representing the allele not under selection.