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Genotype Dependent Interspecific Hybridization of Sorghum bicolor

^a Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX 77840-2474
^b USDA-ARS, Crop Germplasm Research Unit, 430 Heep Center, Texas A&M Univ., College Station, TX 77843-2474
^c Australian Tropical Crops and Forages Collection, Queensland Dep. of Primary Industries and Fisheries, Biloela, QLD, Australia

View larger version (62K): [in a new window]   Fig. 1. Germination of S. nitidum pollen on S. bicolor stigmas and tube growth 24 h after pollination in pistils of S. bicolor genotypes with and without the iap allele. (A) Sorghum nitidum pollen on a stigma of S. bicolor, Iap Iap. Pollen germinated but the pollen tubes seldom grew beyond the stigma branches. (B and C) Sorghum nitidum pollen tubes have grown through the stigma, style, and into the ovary of S. bicolor genotype, iap iap. Scale bars = 1 µm.

View larger version (75K): [in a new window]   Fig. 2. Somatic chromosomes of hybrids between S. bicolor (2n = 2x = 20) and S. angustum (2n = 2x = 10), S. bicolor and S. nitidum (2n = 4x = 20), and S. bicolor and S. macrospermum (2n = 4x = 40). (A) Chromosomes of a hybrid between S. bicolor and S. angustum consisting of five large chromosomes from S. angustum and 10 small chromosomes from S. bicolor. (B) Chromosomes of a S. bicolor x S. nitidum hybrid with 10 large chromosomes from S. nitidum and 10 small chromosomes from S. bicolor. (C) Chromosomes of a S. bicolor x S. macrospermum hybrid with 20 from S. macrospermum and 10 from S. bicolor. The S. bicolor chromosomes are smaller than most of the S. macrospermum chromosomes. Upper arrow shows two chromosomes. Lower arrow shows a chromosome in which the centromere is not fully condensed and appears as a strand. Scale bars = 5 µm.