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Molecular Marker-Assisted Selection for Improved Cooking and Eating Quality of Two Elite Parents of Hybrid Rice

^a Jiangsu Key Lab. of Crop Genetics and Physiology, Key Lab. of Plant Functional Genomics of the Ministry of Education, Agric. College, Yangzhou Univ., Jiangsu 225009, P.R. China
^b Shanghai Institute of Plant Physiology & Ecology, Chinese Academy of Science, Shanghai 200032, P.R. China
^c College of Bioscience & Biotechnology, Yangzhou Univ., Jiangsu 225009, P.R. China

View larger version (25K): [in a new window]   Fig. 1. Comparison of the Wx exon 1-intron 1 junction sequences from rice cultivars with high (H) and intermediate (I) amylose content (AC). Exon and intron sequences are shown in capital and lowercase letters, respectively. The complete sequence is given only for cultivars with high AC, and only those that differ from the corresponding nucleotides are shown in boxes for cultivars with intermediate level of AC. The dashes (–) indicate nucleotide deletions in the intermediate AC cultivar, compared to the corresponding sequence of the high AC cultivar. The bolded sequences represent the recognition site by AccI in the high AC cultivar, where it is not due to the G to T mutation in the case of the intermediate AC cultivar. The primers used for the CAPS marker PCR-AccI determination are underlined.

View larger version (64K): [in a new window]   Fig. 2. Determination of the PCR-AccI molecular marker in individuals of the BC4F2 generation from two target parents, LTF-B (A) and ZS-B (B). Panel (A) lane 1: 9311 (TT genotype); lane 2: LTF-B (GG genotype); lanes 3–12: individuals in BC4F2 generation; and lane 13: hybrid Teyou 559 (GT genotype). Panel (B) lane 1: YH559 (TT genotype); lane 2: ZS-B (GG genotype); lanes 3–12: individuals in BC4F2 generation; and lane 13: Shanyou 63 (GT genotype). Lane M: the 1 kb plus DNA molecular marker (Invitrogen).

View larger version (64K): [in a new window]   Fig. 3. Comparison of Wx gene expression levels among the selected lines and hybrids, and their originals by both transcript (A and B) and protein levels (C). Northern blot analysis of hybridized signals with the Wx probe. The 3.4 kb Wx pre-mRNAs and 2.3 kb Wx mature mRNAs are indicated by arrows (panel A). Panel B shows the rRNAs by EB staining after electrophoresis; SDS-PAGE of Coomassei blue stained Wx proteins purified from the endosperm of mature seeds (panel C). The genotypes of PCR-AccI molecular marker of the analyzed samples is shown in panel D. [Lane 1: LTF-B original; lanes 2–6: five individual plants in BC4F2 generation; lanes 5–6 represent the two selected lines L-tt-1 B and L-tt-3 B; lane 7: 9311; lane 8: ZS-B original; lane 9–10: two selected lines Z-tt-1 B and Z-tt-2 B; lane 11: YH559; lane 12: original hybrid Teyou 63; and lane 13: one new hybrid LTF (tt)-A-1xMH63.]