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Dehydrin-Like Proteins in Soybean Seeds in Response to Drought Stress during Seed Filling

^a Dep. of Crop Production, Jordan Univ. of Science and Technology, Irbid P.O. Box 3030, 22110, Jordan
^b Dep. of Agronomy, Iowa State Univ., Ames, IA 50011

View larger version (12K): [in a new window]   Fig. 1. Three drought stress treatments imposed on soybean plants at beginning seed fill (R5) in the greenhouse experiment. WW: plants were maintained at 80% field capacity (FC) after R5. GS: the soil moisture content was dropped gradually in 7 d after R5 from 80% to 20% FC; then, maintained at 20% FC for 13 d. SS: plants were maintained at 80% FC for 7 d from R5; then, soil moisture content was allowed to drop suddenly in 1 d to 20% FC and maintained for 16 d.

View larger version (26K): [in a new window]   Fig. 2. Average air temperature (a), relative humidity (b), rainfall (c), and soil tensiometer readings at a 45-cm depth (d) for irrigated and nonirrigated conditions recorded after treatments were imposed on soybean plants at beginning of seed fill (R5) in the field experiment.

View larger version (25K): [in a new window]   Fig. 3. Dry weight (DW) and moisture content (MC) of developing soybean seeds harvested from plants exposed to drought stress treatments at beginning seed fill (R5) in the greenhouse and the field experiments. a) Seed dry weight (mg) and moisture content (percentage based on wet weight) of developing soybean seeds from plants exposed to well-watered (WW), gradual stress (GS), and sudden stress (SS) treatments in the greenhouse experiment. b) Seed dry weight (mg) and moisture content (percentage based on wet weight) of developing seeds from plants grown under irrigated (I) and nonirrigated (NI) conditions in the field. Means ± standard error (n = 3–4).

View larger version (76K): [in a new window]   Fig. 4. SDS PAGE of heat-stable proteins for a) developing and b) mature soybean seeds from plants exposed to three drought stress treatments at beginning seed fill (R5) in the greenhouse experiment. WW: well-watered; GS: gradual stress; SS: sudden severe stress treatments. L: large; M: medium; S: small seeds. Equal weight of protein (7 µg) was loaded per lane. Developing seeds were sampled at 2 (R5.2), 4 (R5.4), 10 (R5.6) and 18 d (R5.8) after the stress treatments were imposed at beginning seed fill (R5). The lane on the left side indicates the molecular weights (MW) of standard proteins (SP). The lane on the right is used to specify MW of proteins indicated by arrows.

View larger version (27K): [in a new window]   Fig. 5. Western blot of the heat-stable proteins immunologically reacting with dehydrin antibody for a) developing and b) mature soybean seeds from plants exposed to three drought stress treatments at beginning seed fill (R5) in the greenhouse experiment. WW: well-watered; GS: gradual stress; SS: sudden severe stress treatments. Equal weight of protein (7 µg) was loaded per lane. L: large; M: medium; S: small seeds. The developing seeds were sampled at 2 (R5.2), 4 (R5.4), 10 (R5.6) and 18 d (R5.8) after the stress treatments were imposed at beginning seed fill (R5). The lane on the left side indicates molecular weights (MW) of standard proteins (SP). The lane on the right is used to specify MW of proteins indicated by arrows.

View larger version (51K): [in a new window]   Fig. 6. SDS PAGE of heat-stable proteins for a) developing and b) mature soybean seeds from plants exposed to irrigated (I) and nonirrigated (NI) (rainfed) conditions at beginning seed fill (R5) in the field experiment. Equal weight of protein (7 µg) was loaded per lane. The developing seeds were sampled at 0 (R5), 5 (R5.4), 15 (R5.7), 22 (R6), 43 d (physiological maturity [PM]) after the stress treatments were imposed at beginning seed fill (R5). Mature seeds were sampled at harvest maturity when seeds dried in the field. The lane on the left side indicates the molecular weights (MW) of standard proteins (SP).

View larger version (26K): [in a new window]   Fig. 7. Western blot of heat-stable proteins immunologically reacting with dehydrin antibody for a) developing and b) mature soybean seeds from plants exposed to irrigated (I) and nonirrigated (NI) (rainfed) conditions at beginning seed fill (R5) in the field experiment. Equal weight of protein (7 µg) was loaded per lane. The developing seeds were sampled at 0 (R5), 5 (R5.4), 15 (R5.7), 22 (R6), 43 d (physiological maturity [PM]) after the stress treatments were imposed at beginning seed fill (R5). Mature seeds were sampled at harvest maturity when seeds dried in the field. The lane on the left side indicates the molecular weights (MW) of standard proteins (SP). The lane on the right is used to specify MW of proteins indicated by arrows.