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Published online 8 September 2006
Published in Crop Sci 46:2015-2021 (2006)
© 2006 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Genetic and Molecular Analysis of High Gamma-Tocopherol Content in Sunflower

María J. García-Moreno, Elsa M. Vera-Ruiz, José M. Fernández-Martínez, Leonardo Velasco and Begoña Pérez-Vich*

Instituto de Agricultura Sostenible (CSIC). Apartado 4084. E-14080 Córdoba, Spain


Figure 1
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  		Fig. 1. Histogram of gamma-tocopherol content content (g kg–1 total tocopherols) in an F2 population from the cross between the sunflower lines CAS-12, with standard low gamma-tocopherol content, and IAST-540, with high gamma-tocopherol content.

 

Figure 2
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  		Fig. 2. Amplification products of the SSR marker ORS70. Replicate samples of the low gamma-tocopherol parental line CAS-12, the high gamma-tocopherol parental line IAST-540, the low gamma-tocopherol (G-T) bulk, the high gamma-tocopherol bulk, and four F2 individuals from CAS-12 x IAST-540 are shown.

 

Figure 3
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  		Fig. 3. Molecular map of sunflower linkage group (LG) 8 containing the Tph2 gene determining high gamma-tocopherol content. The ORS and CRT prefixes denote SSR marker loci, and the ZVG prefix denotes INDEL marker loci. The cumulative distances in centimorgans are shown at the left of the map.

 

Figure 4
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  		Fig. 4. Likelihood odds (LODs) for F2 gamma-tocopherol QTL on linkage group (LG) 8 in CAS-12 x IAST-540.

 




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