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Identifying Novel Resistance Genes in Newly Introduced Blast Resistant Rice Germplasm

^a USDA-ARS, Dale Bumpers National Rice Research Center, P.O. Box 1090, Stuttgart, AR 72160-1090
^b Rice Res. & Ext. Ctr., Univ. of Arkansas, 2900 Hwy 130 E, Stuttgart, AR 72160

View larger version (48K): [in a new window]   Fig. 1. Diagram illustrating the location in cM of the 125 SSR markers included in this study and the approximate position of known Pi-genes as reported by Monosi et al. (2004). Marker positions are based on McCouch et al. (2002). The seven markers used to identify blast resistance genes Pi-b, Pi-kh, Pi-ks, Pi-ta (Fjellstrom et al., 2004), and Pi-z (Conaway-Bormans et al., 2003) in U.S. breeding lines are underlined. Following each SSR marker, the number of alleles associated with each SSR marker used in this study and the PIC value (Bolstein et al., 1980) are listed. Markers identified in this study as associated with blast resistance traits (Table 2) are identified in bold italics. The SSR map was designed with MapChart (Voorrips, 2002).

View larger version (41K): [in a new window]   Fig. 2. Genetic distance among 91 blast resistant rice accessions and eleven controls (capitalized) as revealed by UPGMA cluster analysis and Nei (1972). The two main clusters are identified as I and II with sub-clusters denoted with letters (A-F). The controls included ten selected U.S. rice cultivars and Te Qing, which originated in China and is used as a parent in some U.S. breeding programs. Accessions in bold italics were identified as resistant to all blast isolates tested and not having one of the R-genes, Pi-b and/or Pi-ta.