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Published online 18 May 2006
Published in Crop Sci 46:1576-1580 (2006)
© 2006 Crop Science Society of America
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Allelic Frequency at the Rf3 and Rf4 Loci and the Genetics of A3 Cytoplasmic Fertility Restoration in Converted Sorghum Lines

Les C. Kuhlmana, Daryl R. Pringb, William L. Rooneya,* and Hoang V. Tangb

a Dep. of Soil and Crop Sciences, Texas A&M Univ., 2474 TAMU, College Station, TX 77843
b USDA-ARS, Crop Genetics and Environment Research Unit, Dep. of Plant Pathology, Univ. of Florida, Gainesville, FL 32611


Figure 1
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  		Fig. 1. Iodine staining of pollen from hybrids between A3FL3 (Rf3Rf3rf4rf4) and (A) IS1112C (Rf3Rf3Rf4Rf4), (B) SC512 (Rf3Rf3Rf4Rf4), and (C) SC214 (rf3rf3Rf4Rf4) and between A3FL4 (rf3rf3Rf4Rf4) and (D) IS1112C, (E) SC512, and (F) SC214. All show approximately 50% fully stainable pollen except C (25%) and F (0%).

 

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  		Fig. 2. Northern transcript analyses of sorghum lines probed with orf107 clone pHC104 (Tang et al. 1996b). A, SC192; B, SC215; C, SC235; D, SC306; E, SC512; F, A3FL4/SC215; G, A3FL4/SC235; H, A3FL4/SC192; I, A3FL4/SC214; J, A3FL4/SC512; K, A3FL4/SC306; L, A3FL4/SC315; M, A3Tx398; N, IS1112C. Transcripts of 1110, 870, and 810 nt represent intact orf107 transcripts, while the 380 nt transcript represents product of enhanced orf107 transcript processing. The 650 nt transcript is derived from atp9, detected by virtue of atp9 sequences in the orf107 chimeric configuration.

 




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