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Marker-Assisted Selection for the Broad-Spectrum Potato Late Blight Resistance Conferred by Gene RB Derived from a Wild Potato Species

Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA

View larger version (50K): [in a new window]   Fig. 1. Development of an RB-specific PCR marker. (A) Locations of six pairs of primers within the RB gene. Most of the primers are located within the leucine-rich repeat (LRR) domain in the second exon. (B) Alignment of the primer set no. 1 sequences in the corresponding regions of RB, rb and three RGAs. The exact primer sequences are highlighted in gray.

View larger version (95K): [in a new window]   Fig. 2. Detection of the RB gene using the RB-specific PCR marker in plasmids or plants (transgenic Katahdin lines or backcrossed progenies derived from potato x Solanum bulbocastanum somatic hybrids) that are known to either have or not have the RB gene. Lane 1, potato cultivar Katahdin; Lane 6, negative control without template DNA; Lane 22, S. bulbocastanum clone PT29; Lanes 2, 7, 12, 25, 27, plasmids containing RGA1, RGA4, rb, RB, RGA3, respectively; Lane 3, 4, 11, 13, 17, 18, 21, 26, late blight–resistant transgenic Katahdin lines SP922, SP968, SP925, SP960, SP1006, SP1464, SP898, SP920, respectively; Lane 8, 9, 16, 19, 23, late blight–susceptible transgenic Katahdin lines SP937, SP941, SP991, SP947, SP953, respectively; Lane 5, 10, 15, 24, late blight–resistant BC1 clones J101A32, J101A45, J101A7, J101K6, respectively; Lane 14, 20, late blight–susceptible BC1 clones J101A39 and J101A20, respectively; Lane 28, late blight–resistant BC2 clone J101K6A22. A 213-bp band is amplified in all lanes that contain the RB gene.

View larger version (110K): [in a new window]   Fig. 3. Greenhouse evaluation of late blight resistance of breeding lines derived from potato x Solanum bulbocastanum somatic hybrids. The plants were challenged with Phytophthora infestans isolate US930287. Photos were taken 10 d after inoculation. A, a resistant line WTS1269–3; B, a susceptible line WTS1258–4; C, a resistant line 4H5124–2; D, a susceptible line 4H5133–6.