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Published online 24 January 2006
Published in Crop Sci 46:234-242 (2006)
© 2006 Crop Science Society of America
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Intersubgenomic Heterosis in Rapeseed Production with a Partial New-Typed Brassica napus Containing Subgenome Ar from B. rapa and Cc from Brassica carinata

Maoteng Li, Xin Chen and Jinling Meng*

National Key Lab. of Crop Improvement, Huazhong Agricultural Univ., Wuhan 430070, China


Figure 1
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Fig. 1. The variation of chromosome numbers in plants derived from pentaploid hybrids (AnArBcCnCc).

 

Figure 2
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Fig. 2. Fertility characteristics of new-typed B. napus. (a) A PMC with 19 bivalents from one of 38-chromosomed plant family, 1SH. (b) A PMC with univalents in one partial new-typed B. napus of 1SH family (arrow shows the univalent). (c) Developing siliques from another 38-chromosomed family, GXX, showing normal seed setting.

 

Figure 3
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Fig. 3. Identification of new-typed B. napus with AFLP markers. Lanes from left to right: B. nigra var. Juntus (BbBb), B. oleracea var. Sucheng 1 (C°Co), B. rapa var. Shiqian Baiyoucai (S) (ArAr), B. carinata var. 10167 (BcBcCcCc); and B. napus var. Hua Shuang 3 (H) (AnAnCnCn) and individual plants of partial new-typed B. napus (Ar/An/Cn/Cc) came from a F3 family derived from 1SH, an interspecific cross, (B. carinata var. 10167 x B. rapa var. Shiqian Baiyoucai) x B. napus var. Hua Shuang 3. The subgenomic specific bands were shown with blank arrow (Ar), coattail arrow (Bc), and quinquangular arrow (Cc), respectively. AFLP markers were amplified with the primer pair E+AGT/M+CTC.

 

Figure 4
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Fig. 4. Intersubgenomic hybrids growing at different stages. (a) Seedlings. Left two, a intersubgenomic hybrid Xiangyou 15 x GXX-5; right two, natural B. napus, elite cultivar Zhongyou 821 as standard control. (b) The intersubgenomic hybrid of Xiangyou 15 x GXX-5 at flowering stage (blank arrow and coattail arrow represent Zhongyou 821 and intersubgenomic hybrid, respectively).

 

Figure 5
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Fig. 5. Frequency distribution of partially intersubgenomic hybrids on seed yield. Curve I and II represent the seed yield of hybrids that were made with partial new-typed B. napus in F3 and F4 generations separately and sown in 2000 and 2001, respectively. The asterisk on the curve shows the seed yield of Zhongyou 821.

 

Figure 6
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Fig. 6. The scatter plot of intersubgenomic hybrids derived from crosses between natural B. napus cultivar Huanshuang 3 and plants of 1SH, partial new-typed B. napus.

 





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