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Published online 23 September 2005
Published in Crop Sci 45:2355-2362 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Molecular and Genetic Characterization of Nicotiana glutinosa L. Chromosome Segments in Tobacco mosaic virus-Resistant Tobacco Accessions

R. S. Lewis*, S. R. Milla and J. S. Levin

Dep. of Crop Science, North Carolina State Univ., Campus Box 7620, Raleigh, NC 27695



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  		Fig. 1. PCR amplification of nuclear DNA from 18 lines of tobacco using the N-gene specific primer pairs (a) E1 + E2 and (b) N1 + N2. Lanes 1 through 20 are: (1) 100 bp ladder, (2) Samsun, (3) Red Russian, (4) Connecticut Broadleaf, (5) K326, (6) NC 1125-2, (7) TI 1407, (8) TI 1459, (9) 1462, (10) TI 1463, (11) TI 1473, (12) TI 1492, (13) TI 1500, (14) TI 1501, (15) TI 1504, (16) Xanthi nc, (17) Xanthi NN, (18) Samsun NN, (19) Holmes Samsoun, (20) 100-bp ladder.

 


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  		Fig. 2. AFLP genotypes for accessions and selected cultivars. Lines 2–9 carry the N gene on chromosome H; Lines 10–16 carry the N gene on an alternative chromosome; Lines 17 and 18 are TMVS cultivars. Presence of AFLP marker is indicated by shading. Genotypes are provided here for only 19 of 168 AFLP markers. The 149 markers not shown were all present for Holmes Samsoun and absent for the remaining accessions and cultivars.

 


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  		Fig. 3. Linkage maps generated using progeny from three BC1F1 families. Map distances are in centimorgans (cM). (A) TI 1462/K326//K326 BC1F1; (B) Samsun NN/K326//K326 BC1F1; (C) TI 1500/K326//K326 BC1F1. M1 = AFLP marker E35M50-264; M2 = E45M56-75; M3 = E32M61-293; M4 = E45M55-82; M5 = E36M58-333; M6 = E46M49-323; M7 = E45M54-393; M8 = E33M55-219; M9 = E45M59-240; M10 = E38M50-404; M11 = E35M8-317; M12 = E45M49-364; M14 = E36M58-100; M15 = E35M61-522; M16 = E38M49-328.

 




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