Crop Science
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Published online 23 September 2005
Published in Crop Sci 45:2346-2354 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Abstract Freely available
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Milla, S. R.
Right arrow Articles by Rufty, R. C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Milla, S. R.
Right arrow Articles by Rufty, R. C.
Agricola
Right arrow Articles by Milla, S. R.
Right arrow Articles by Rufty, R. C.
Related Collections
Right arrow Tobacco
Right arrow Crop Genetics

RAPD and SCAR Markers Linked to an Introgressed Gene Conditioning Resistance to Peronospora tabacina D.B. Adam. in Tobacco

S. R. Millaa, J. S. Levina, R. S. Lewisa,* and R. C. Ruftyb

a Dep. of Crop Science, North Carolina State Univ., Campus Box 7620, Raleigh, NC 27695-7620
b Office of the Graduate School, North Carolina State Univ., Campus Box 71-2, Raleigh, NC 27695



View larger version (19K):

[in a new window]
 
Fig. 1. Development of lines or populations utilized for identification of markers linked to blue mold resistance. Three cycles of doubled haploid breeding were used to develop burley tobacco germplasm possessing blue mold resistance derived from Ovens 62. Selected Cycle 1 and Cycle 2 DH lines were used to generate the second and third cycles, respectively. NC-BMR 42 and NC-BMR 90 were Cycle 1 selections released as germplasm lines by the North Carolina Agricultural Research Service. NC-BMR 113 and NC-BMR 114 were Cycle 2 selections that were also released as germplasm lines.

 


View larger version (26K):

[in a new window]
 
Fig. 2. Histograms showing frequency distributions for blue mold resistance of doubled haploid lines evaluated in experiments BM1, BM2, BM3, and BM4. Ratings for resistant check cultivar Ovens 62 and susceptible check cultivar Ky 14 are indicated by arrows.

 


View larger version (14K):

[in a new window]
 
Fig. 3. QTL map for chromosome segment associated with blue mold resistance produced using interval mapping. Markers are indicated beneath the X axis with intervals between them indicated in centimorgans immediately above the X axis. The 1 – LOD confidence interval for the position of the QTL is indicated by the bold horizontal line above the X axis. The experiment-wise LOD threshold significance level determined using 1000 permutations of the procedure of Churchill and Doerge (1994) as implemented by WinQTLCart is indicated by the horizontal line crossing the Y axis at LOD = 1.23.

 


View larger version (43K):

[in a new window]
 
Fig. 4. (a) SCAR marker SOPR06.268, (b) SCAR marker SUBC180.251. Lane 1 = molecular size marker, Lane 2 = water, Lanes 3–9 are blue mold susceptible varieties Ky 14, Ky 17, TN 86, TN 90, McNair 944, Speight G-70, and Speight G-28. Lanes 10–14 are blue mold resistant lines Ovens 62, BMR 113, BMR 114, NC BMR 42, and NC BMR 90.

 





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education
Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality
The Plant Genome
Copyright © 2005 by the Crop Science Society of America.