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RAPD and SCAR Markers Linked to an Introgressed Gene Conditioning Resistance to Peronospora tabacina D.B. Adam. in Tobacco

^a Dep. of Crop Science, North Carolina State Univ., Campus Box 7620, Raleigh, NC 27695-7620
^b Office of the Graduate School, North Carolina State Univ., Campus Box 71-2, Raleigh, NC 27695

View larger version (19K): [in a new window]   Fig. 1. Development of lines or populations utilized for identification of markers linked to blue mold resistance. Three cycles of doubled haploid breeding were used to develop burley tobacco germplasm possessing blue mold resistance derived from Ovens 62. Selected Cycle 1 and Cycle 2 DH lines were used to generate the second and third cycles, respectively. NC-BMR 42 and NC-BMR 90 were Cycle 1 selections released as germplasm lines by the North Carolina Agricultural Research Service. NC-BMR 113 and NC-BMR 114 were Cycle 2 selections that were also released as germplasm lines.

View larger version (26K): [in a new window]   Fig. 2. Histograms showing frequency distributions for blue mold resistance of doubled haploid lines evaluated in experiments BM1, BM2, BM3, and BM4. Ratings for resistant check cultivar Ovens 62 and susceptible check cultivar Ky 14 are indicated by arrows.

View larger version (14K): [in a new window]   Fig. 3. QTL map for chromosome segment associated with blue mold resistance produced using interval mapping. Markers are indicated beneath the X axis with intervals between them indicated in centimorgans immediately above the X axis. The 1 – LOD confidence interval for the position of the QTL is indicated by the bold horizontal line above the X axis. The experiment-wise LOD threshold significance level determined using 1000 permutations of the procedure of Churchill and Doerge (1994) as implemented by WinQTLCart is indicated by the horizontal line crossing the Y axis at LOD = 1.23.

View larger version (43K): [in a new window]   Fig. 4. (a) SCAR marker SOPR06.268, (b) SCAR marker SUBC180.251. Lane 1 = molecular size marker, Lane 2 = water, Lanes 3–9 are blue mold susceptible varieties Ky 14, Ky 17, TN 86, TN 90, McNair 944, Speight G-70, and Speight G-28. Lanes 10–14 are blue mold resistant lines Ovens 62, BMR 113, BMR 114, NC BMR 42, and NC BMR 90.