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Barley HVA1 Gene Confers Salt Tolerance in R3 Transgenic Oat

Dep. of Crop and Soil Sciences, Michigan State Univ., Plant and Soil Science Bldg., East Lansing, MI 48824

View larger version (129K): [in a new window]   Fig. 1. R3 transgenic seedlings after in vitro selection.

View larger version (23K): [in a new window]   Fig. 2. PCR amplification of the bar (0.59 kb) and HVA1 (0.7 kb) genes shows the presence of the transgenes in R3 for the five lines. Lane 1: 100-bp ladder marker, Lane 2: plasmid (positive control), Lane 3: nontransformed (negative control), and Lanes 4–8: transgenic lines.

View larger version (42K): [in a new window]   Fig. 3. GUS expression in R3 transgenic oat seed husks (a), seeds (b), and root segments (c)

View larger version (27K): [in a new window]   Fig. 4. Top; pBY520 partial map, bottom; Southern blot (a) and Northern blot (b) analyses showing bands for R3 of transgenic oat plants digested with HindIII, M: ladder marker; P: plasmid BY520; Lanes 1–5: Ogle BRA-82, Ogle BRA-17, Ogle BRA-8, Ogle BRA-19, Ogle BRA-41 respectively; C: nontransgenic control.

View larger version (40K): [in a new window]   Fig. 5. The effect of increasing NaCl concentrations on number of days to heading (a), plant height (b), flag leaf area (c), root length (d), panicle length (e), and number of spikelets/panicle (f) for the transgenic lines and the nontransgenic control.

View larger version (48K): [in a new window]   Fig. 6. R3 transgenic oat (a) and nontransgenic (b) at 150 mM NaCl stress.

View larger version (37K): [in a new window]   Fig. 7. R3 transgenic (a) and nontransgenic (b) oat roots at 100 mM NaCl stress.