HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Toda, K. Articles by Takahashi, R. Search for Related Content PubMed Articles by Toda, K. Articles by Takahashi, R. Agricola Articles by Toda, K. Articles by Takahashi, R. Related Collections Soybean Temperature Stress

Structure of Flavonoid 3'-Hydroxylase Gene for Pubescence Color in Soybean

^a National Institute of Crop Science and University of Tsukuba, Kannondai 2-1-18, Tsukuba, Ibaraki, 305-8518 Japan
^b National Agric. Research Center, Tsukuba, Ibaraki, 305-8666 Japan
^c National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan

View larger version (17K): [in a new window]   Fig. 1. Structure of flavonoid 3'-hydroxylase gene in soybean near-isogenic line, To7B. The entire F3'H gene is covered by two genomic clones, F3'H-G1 and F3'H-G2. PCR primer positions are indicated by small arrows. Designation of the primers is shown to the left of each primer pair. Information of the primers is presented in Table 1. The F3'H gene consists of three exons and two introns.

View larger version (11K): [in a new window]   Fig. 2. Motifs found in the promoter region of F3'H gene in soybean near-isogenic line, To7B. The numbers refer to the location of motifs in the DNA sequence where the A of the start codon is numbered as 1.

View larger version (100K): [in a new window]   Fig. 3. SSR band polymorphism of soybean near-isogenic lines for pubescence color, To7B (TT) and To7G (tt), and cultivars with tawny pubescence (TT, Karafuto-1, Moshidou Gong 503, and Clark) and cultivars with gray pubescence (tt, Toyosuzu, Misuzudaizu, and Harosoy) using the SSR primer, SoyF3'H. The migration of size markers is shown to the right of the gel.

View larger version (45K): [in a new window]   Fig. 4. SSR band polymorphisms of a F2 population derived from a cross between a tawny pubescence cultivar Karafuto-1 (TT) and a gray pubescence cultivar Toyosuzu (tt) using the SSR primer SoyF3'H. Genotypes at the T locus were determined by testing 15 progeny of each F3 families. M, molecular size marker; 1, Karafuto-1; 2, Toyosuzu; T, F2 plants with TT genotype; H, F2 plants with Tt genotype; t, F2 plants with tt genotype. The SSR bands cosegregated with the genotype at the T locus. The migration of size markers is shown to the right of the gel.