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Published online 26 August 2005
Published in Crop Sci 45:1985-1989 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Inexpensive, High Throughput Microplate Format for Plant Nucleic Acid Extraction

Suitable for Multiplex Southern Analyses of Transgenes

L. Flagela, J. R. Christensena, C. D. Gustusa, K. P. Smitha, P. M. Olhofta,b, D. A. Somersa and P. D. Matthewsa,c,*

a Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108
b BASF Plant Science, 26 Davis Drive, Research Triangle Park, NC 27709
c Crop Improvement, S.S. Steiner, Inc., 655 Madison Ave, New York, NY 10021



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  		Fig. 1. Extracted DNA from various plants is of high molecular weight and susceptible to endonuclease digestion. Agarose gels stained with ethidium bromide: (A) barley undigested, close-up; (B) hop, undigested, close-up; (C) hop pellets, degraded in food processing, close-up; (D) tobacco, complete set of 96 extractions separated on a two-tiered gel, HindIII; (E) soybean, complete set of 96 on a two-tiered gel, HindIII. Molecular weight markers: 1 kb ladder (Invitrogen, Carlsbad, CA), except B and D, {lambda} Hind III markers.

 


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  		Fig. 2. Example of soybean Southern detection of primary transformants probed with the Hpt (hygromycin phosphotransferase) gene.

 




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