HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Ratnayaka, I. Articles by Chibbar, R. N. Search for Related Content PubMed Articles by Ratnayaka, I. Articles by Chibbar, R. N. Agricola Articles by Ratnayaka, I. Articles by Chibbar, R. N. Related Collections Wheat Cell Biology & Molecular Genetics Plant Genetic Resources

Construction and Characterization of a BAC Library of a Cold-Tolerant Hexaploid Wheat Cultivar

Dep. of Plant Sciences, Univ. of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada

View larger version (41K): [in a new window]   Fig. 1. Determination of organelle DNA contamination present in the Norstar BAC library. Hybridization signals obtained from high-density filters carrying 18432 double-spotted clones after hybridization to 32P-labeled chloroplast rbcL and psbA (a) and mitochondrial cox1 (b) probes. Positive clones are identified by a duplicated signal pattern.

View larger version (93K): [in a new window]   Fig. 2. Determination of BAC clone insert sizes. (a) PFGE analysis of fragments generated from NotI digestion of 18 BAC DNA samples. Low Range PFG marker was used as size standard (M). (b) Histogram showing distribution of insert sizes determined from analysis of 119 BAC clones.

View larger version (89K): [in a new window]   Fig. 3. Screening of the BAC library using gwm18 SSR marker. Agarose gel electrophoresis of PCR amplification products obtained from screening the entire library of 69 pools. The six pools showing the expected 175-bp fragment for gwm18 are indicated by vertical arrows. Lanes of samples containing Norstar genomic DNA (N), no DNA (C), and 100 bp ladder (M) are indicated.

View larger version (95K): [in a new window]   Fig. 4. Identification of BAC clones carrying highly-repetitive DNA sequences. (a) PFGE analysis of 15 BAC clones digested with NotI. Low Range PFG (M) was used as size standard. (b) Autoradiogram of gel in (a) after blotting to Hybond-N+ membrane and hybridization to 32P-labeled Norstar nuclear DNA.

View larger version (94K): [in a new window]   Fig. 5. Stability test of BAC clones carrying repetitive DNA. HindIII fingerprints produced from plasmid DNA extracted from five BAC clones after 1, 2, 3, 4, and 5 d of growth. Low Range PFG marker (M) was used as size standard.