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Root Temperature and Aeration Effects on the Protein Profile of Canola Leaves

^a Dep. of Forestry, Wildlife and Fisheries, 274 Ellington Plant Sciences Bldg., Univ. of Tennessee, Knoxville, TN 37996-4563
^b Dep. of Agricultural, Food and Nutritional Science, Univ. of Alberta, Edmonton, AB, Canada T6G 2P5
^c Dep. of Biological Sciences, Univ. of Calgary, Calgary, AB, Canada T2N 1N6

View larger version (22K): [in a new window]   Fig. 1. (a) Shoot and root fresh weights and (b) leaf area of canola plants treated for 12 d with roots aerated at 24/18°C (day/night; control) or 10/10°C (cold), or not aerated at 24/18°C (hypoxic) or 10/10°C (cold + hypoxic). Standard errors are indicated. n = 16.

View larger version (19K): [in a new window]   Fig. 2. Midday transpiration rates of canola plants treated for 12 d with roots aerated at 10/10°C (day/night), or nonaerated at 24/18 or 10/10°C. Upper and lower limits of control (24/18°C) 95% confidence interval are shown. n = 12.

View larger version (28K): [in a new window]   Fig. 3. Shoot and root concentrations of (a) total soluble sugars and (b) starch of canola plants treated for 12 d with roots aerated at 24/18°C (day/night; control) or 10/10°C (cold), or not aerated at 24/18°C (hypoxic) or 10/10°C (cold + hypoxic). Standard errors are indicated. n = 14.

View larger version (112K): [in a new window]   Fig. 4. Sodium dodecyl sulfate (13%)–polyacrylamide gel electrophoresis gel images showing the protein profiles of (A) the control, and (B, C, D) the treated (hypoxic, cold, and hypoxic + cold, respectively) canola samples. The numbers indicate the protein spots that were reproducibly affected by exposure to the stresses.