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Stability and Activity of the Major Nod Factor Produced by Bradyrhizobium japonicum following Purification, Sterilization, and Storage

^a Dep. Agronomy, Fac. Agric. Univ., Bengkulu, Indonesia
^b Dep. of Plant Science, McGill Univ., Macdonald Campus, 21111 Lakeshore Road, Ste. Anne de Bellevue, QC, Canada H9X 3V9

View larger version (34K): [in a new window]   Fig. 1. The effects of container material for lyophilization (A) and filter type for filtration (B) on NodBj-V(C18:1 MeFuc) recovery. Each value is a mean ± S.E. (n = 4). Glass or polypropylene (PP) was used for the freeze-drying experiment; cellulose acetate (CA), mixed cellulose ester (MCE), nylon (N), polyestersulfone (PES), or polytetrafluoroethylene (PTFE) was used for filtration.

View larger version (19K): [in a new window]   Fig. 2. The effect of storage temperature on NodBj-V(C18:1 MeFuc) recovery. Each value is a mean ± S.E. (n = 4).

View larger version (25K): [in a new window]   Fig. 3. The effect of autoclaving for 30 min and storage at 4 and 23°C for 8 and 16 mo on percentage of seed germination over a 70-h period. Each value is a mean ± S.E. (n = 10 with 10 seeds each).