HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by Vaughn, T. Articles by Pershing, J. Search for Related Content PubMed Articles by Vaughn, T. Articles by Pershing, J. Agricola Articles by Vaughn, T. Articles by Pershing, J. Related Collections Maize Insect Resistance Cell Biology & Molecular Genetics

A Method of Controlling Corn Rootworm Feeding Using a Bacillus thuringiensis Protein Expressed in Transgenic Maize

Monsanto Company, 700 Chesterfield Parkway West, Chesterfield, MO 63017

View larger version (27K): [in a new window]   Fig. 1. Plasmid map of PV-ZMIR13 and the inserted DNA in event MON863. (A) The MluI restriction fragment of plasmid PV-ZMIR13 was used for transformation to generate event MON863. This region is highlighted by the curved line while the remaining section is the plasmid backbone and elements therein used for bacterial selection. (B) A map of the characterized transgene insert in event MON863. The arrows depict the directionality of the genetic elements within the insert.

View larger version (56K): [in a new window]   Fig. 2. Southern blot analysis for insert and copy number. Ten micrograms of MON846 DNA (A1xA634) (lane 1) and MON863 DNA (Lanes 2 and 5) were digested with NdeI (Panel A) or EcoRV (Panel B). Plasmid PV-ZMIR13 DNA (Lanes 3 and 4) was spiked into 10 µg of MON846 DNA and digested with NdeI and EcoRV (Panel A) or EcoRV (Panel B). High molecular weight DNA ladder (Gibco BRL, Gaithersburg, MD) was loaded on the long run and molecular weight markers II and IX (Roche, Indianapolis, IN) were loaded on the short run in both panels. The arrows denote the sizes obtained from the molecular weight markers on the ethidium-stained gel.

View larger version (63K): [in a new window]   Fig. 3. Southern blot analysis for nptII and cry3Bb1 cassette and coding region intactness and plasmid PV-ZMIR13 backbone. Ten micrograms of MON846 DNA (A1xA634) (lane 1) and MON863 DNA (Lanes 2 and 5) were digested with HindIII. Plasmid PV-ZMIR13 DNA (Lanes 3 and 4) was spiked into 10 µg of MON846 DNA and digested with HindIII. The membranes were probed with 32P-labeled full-length nptII coding sequence (Panel A), full-length cry3Bb coding sequence (Panel B), or two backbone probes encompassing the entire backbone sequence except for the nptII coding region (Panel C). High molecular weight DNA ladder (Gibco BRL, Gaithersburg, MD) was loaded on the long run and molecular weight markers II and IX (Roche, Indianapolis, IN) were loaded on the short run in all three panels. The arrows denote the sizes obtained from the molecular weight markers on the ethidium-stained gel.

View larger version (184K): [in a new window]   Fig. 4. Damage inflicted by CRW larval feeding on MON863 hybrids and conventional corn hybrids. Figure 4a shows the level of plant stunting on the conventional corn hybrid (left three rows) compared to MON863 protected hybrids (right three rows). In Fig. 4b, the left root is a conventional corn hybrid and has been severely damaged while the root on the right side of the frame is protected by event MON863.