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Published online 28 March 2005
Published in Crop Sci 45:832-839 (2005)
© 2005 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Can Lignin Be Accurately Measured?

Ronald Hatfielda,* and Romualdo S. Fukushimab

a USDA-ARS US Dairy Forage Research Center, 1925 Linden Drive West, Madison, WI 53706
b Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Duque de Caxias-Norte, 225, 13635-900, Pirassununga, SP, Brazil



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  		Fig. 1. Reaction mechanism for thio derivatization of lignin by thioglycolate reagent to render lignin soluble under alkaline conditions.

 


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  		Fig. 2. Reaction mechanism for acetyl derivatization of lignin by acetyl bromide reagent to render lignin soluble under acidic conditions.

 




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