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EMBRYOGENIC CALLUS INDUCTION AND REGENERATION IN A PENTAPLOID HYBRID BERMUDAGRASS CV. TIFTON 85

^a Agronomy Dep., Univ. of Florida, Gainesville, FL 32611-0300
^b Agronomy Dep., Plant Molecular and Cellular Biology Program, and Genetics Institute, Univ. of Florida, Gainesville, FL 32611-0300
^c Range Cattle Research and Education Center, Univ. of Florida, 3401 Experiment Station, Ona, FL 33865-9706

View larger version (184K): [in a new window]   Fig. 1. Callus induction and regeneration from immature inflorescence explants of hybrid bermudagrass Tifton 85. (a) Callus initiated from immature inflorescence (less than 3 cm) explants of Tifton 85 cultured on MS medium containing 30 g L–1 sucrose, 4 mg L–1 2,4-D and 0.01 mg L–1 BAP for 4 wk in the dark. (b) Initial shoot regeneration on the same medium after 3 wk under a 16-h light regimen. (c) Regenerated plants of Tifton 85 rooted on hormone-free MS medium. (d) Regenerated plants of Tifton 85 after acclimatization in the greenhouse.