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Determination of Ergot Alkaloid Content in Tall Fescue by Near-Infrared Spectroscopy

^a Dep. of Agron., Univ. of Missouri, Columbia, MO 65211
^b Dep. of Crop and Soil Sci., Univ. of Georgia, Athens, GA 30602
^c College of Veterinary Medicine, Univ. of Missouri, Columbia, MO 65211

View larger version (16K): [in this window] [in a new window]   Fig. 1. Strategy used to develop near-infrared spectroscopic calibrations to measure total ergot alkaloid content in tall fescue.

View larger version (13K): [in this window] [in a new window]   Fig. 2. Ergovaline as quantified by high performance liquid chromatography (HPLC) vs. total ergot alkaloid content (relative concentration) determined by immunochemistry. Samples were stockpiled tall fescue infected with no endophyte, an introduced endophyte, and a toxic endophyte. Abs, absorbance.

View larger version (21K): [in this window] [in a new window]   Fig. 3. Three-dimensional hypersphere plot of the first three principle component scores calculated from near-infrared reflectance data of 84 diverse tall fescue samples. The plot has been rotated to present maximum separation between two files—those including scores of stockpiled tall fescue (+) and those containing scores of the remaining population ().

View larger version (20K): [in this window] [in a new window]   Fig. 4. Actual and predicted ergot alkaloid content in stockpiled tall fescue infected with a toxic endophyte. Actual absorbance, which is negatively correlated with ergot alkaloid concentration, was quantified by immunochemistry. Predicted absorbance was by near-infrared spectroscopy. Abs, absorbance.