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Published online 23 February 2005
Published in Crop Sci 45:717-721 (2005)
© 2005 Crop Science Society of America
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Introgression of Perennial Teosinte Genome into Maize and Identification of Genomic In Situ Hybridization and Microsatellite Markers

Qilin Tanga, Tingzhao Ronga,*, Yunchun Songb, Junpin Yangc, Guangtang Pana, Wanchen Lia, Yubi Huanga and Moju Caoa

a Maize Research Inst., Sichuan Agriculture Univ., Ya'an, Sichuan 625014, P.R. China
b The Key Lab. of the Ministry of Education of Plant Developmental Biology, Wuhan Univ., Wuhan, 430072, P.R. China
c Sichuan Crop Research Inst., Chengdu, 625000, P.R. China


Figure 1
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  		Fig. 1. The morphological characters of wild parent and its interspecific hybrid progenies. (A) Perennial teosinte, (B) the F1 of maize x perennial teosinte, (C) the F2 of maize x perennial teosinte, (D) the BC1F3 of maize x perennial teosinte (MZI202).

 

Figure 2
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  		Fig. 2. Multicolor GISH images of the mitotic metaphase chromosomes of the parent maize and MZI202. Their chromatins were probed by the probes mixture containing the digoxigenin-labeled maize genomic DNA and the biotin-labeled perennial teosinte genomic DNA, and the nuclei were counterstained with DAPI (blue). (A) The metaphase chromosomes of maize showing strong yellow signals or yellow-green band signals, red arrows showing maize chromosome 6. (B) 20 metaphase chromosomes of MZI202, red arrow showing one maize chromosome 6 in MZI202. (C, D) the metaphase chromosomes of MZI202, the arrows showing three perennial teosinte chromosomes with red signals.

 

Figure 3
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  		Fig. 3. Identification of substituted maize chromosomes by SSRs. (C) The PCR products of genomic DNA of MZI202 plant, (A) maize, and (B) perennial teosinte were shown after electrophoresis in 4.5% (w/v) agarose gel. The arrows showed the specific polymorphism where one member of chromosome 6 and two members of chromosome 10 were possibly replaced by perennial teosinte chromosomes in MZI202.

 




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