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Assessment of Inter Simple Sequence Repeat Markers to Differentiate Sympatric Wild and Domesticated Populations of Common Bean

^a Dep. of Agronomy and Range Science, Univ. of California, 1 Shields Ave., Davis, CA 95616-8515, USA
^b Current address: CSIRO Plant Industry, Horticulture Unit, PMB 44, Winnellie 0822, Darwin, NT, Australia
^c Inst. de Biología, Univ. Nacional Autónoma de México, México, DF, México
^d Dipartimento di Scienze degli Alimenti, Univ. Politecnicà delle Marche, Ancona, Italy

View larger version (146K): [in this window] [in a new window]   Fig. 1. Inter simple sequence repeat electrophoretic assessment of genetic diversity in common bean. A gel is shown in which several individuals showed one or more fragments (arrowheads on the right side of the gel) that were atypical for the populations (B, Beige; NB, Negro Brillante; NO, Negro Opaco; W, wild) after amplification using primer (GACA)3RT.

View larger version (50K): [in this window] [in a new window]   Fig. 2. Distribution of inter simple sequence repeat (ISSR) markers among linkage groups of the recombinant inbred population BAT93 x Jalo EEP558. No ISSRs mapped to linkage groups B4 and B9. Only ISSR loci are shown, in addition to two previously mapped markers (Freyre et al., 1998) on each linkage group to orient them with respect to other common bean maps. The ISSR locus names include the primer sequence followed after the period by the size of the scored band in bp. Markers in bold were mapped with a log-likelihood ratio above 3, those in normal type with a log-likelihood ratio below 3. Distances to the left of each linkage group are expressed in Kosambi cM. Linkage groups were not drawn to scale.

View larger version (26K): [in this window] [in a new window]   Fig. 3. Dendrogram generated by unweighted pair group method with arithmetic means based on Dice (1945) coefficients. The clusters correspond to the populations, indicated at the right of the dendrogram. The mixed cluster includes individuals from the Negro Brillante (NB), Negro Opaco (NO), and wild (W) populations (see text for composition). Individuals listed at the right constitute exceptions to the clustering into the Beige, Negro Brillante, Negro Opaca, Rojo, and wild populations. All 170 haplotypes are shown in this figure, although some haplotypes were observed in more than one individual.

View larger version (26K): [in this window] [in a new window]   Fig. 4. Frequency distribution of markers within each of the five populations analyzed (B, Beige; NB, Negro Brillante; NO, Negro Opaco; R, Rojo; W, wild). The bimodal nature of the distributions reflects the codominant nature of the inter simple sequence repeat markers.