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Soybean Allergenicity and Suppression of the Immunodominant Allergen

Plant Genetics Unit, USDA/ARS, Donald Danforth Plant Science Center, St. Louis, MO 63132

View larger version (117K): [in this window] [in a new window]   Fig. 1. Demonstration of the atopic skin response of a swine previously sensitized with peanut and soybean proteins. Photograph courtesy of Agricultural Research Magazine.

View larger version (98K): [in this window] [in a new window]   Fig. 2. The sequence alignment of four allergenic members of the papain family of cysteine proteases is shown.

View larger version (90K): [in this window] [in a new window]   Fig. 3. A SDS/PAGE immunoblot of total soybean proteins (top panel) probed with a monoclonal antibody against P34/Gly m Bd 30k (middle panel) and sera from a human baby with soybean sensitivity (bottom panel) is shown. These blots show that P34/Gly m Bd 30k is the primary or immunodominant allergen.

View larger version (138K): [in this window] [in a new window]   Fig. 4. The suppression of P34 by sense cosuppression is shown in the adjacent blots. The three panels show replicate samples stained for total protein with amido black, and labeled with either anti-P34 monoclonal antibody or with IgEs from a soybean-sensitive person. Lane 1 is a transgenic control of another soybean suppressing the /' conglycinin (arrowhead) that does contain P34 (Kinney et al., 2001). Lane 2 is a transgenic soybean line containing a construct that includes the P34 cDNA that did not induce suppression. Lane 3 is a T3 generation homozygous transgenic soybean with the P34 construct inducing the complete suppression of P34 accumulation. Note the complete absence of P34 assayed by the monoclonal antibody and IgE cross-reactivity using sera from a soybean-sensitive person. Reprinted from Plant Physiol. 132: 36-43 (2003).